Andrew L Darrow, Age 659318 Kendale Rd, Potomac, MD 20854

6420157, Jul 16, 2002

Aug 31, 1999

09/386642

Andrew Darrow - Lansdale PA
Jenson Qi - Branchburg NJ

Ortho-McNeil Pharmaceutical, Inc. - Raritan NJ

C12N 964

435226, 435 697, 435 23, 435174, 4352523, 4353201, 424 9464, 536 232, 536 234

We describe the DNA sequences encoding an expression vector system that will permit, through limited proteolysis, the activation of expressed zymogen precursor of (S1) serine proteases in a highly controlled and reproducible fashion. The processed expressed protein, once activated, is rendered in a form amenable to measuring the catalytic activity. This catalytic activity of the activated form, is often a more accurate representation of the mature S1 protease gene product relative to the unprocessed zymogen precursor. Thus, this series of zymogen activation constructs represents a significant system for the analysis and characterization of serine protease gene products.

6426199, Jul 30, 2002

Aug 31, 1999

09/386629

Andrew Darrow - Lansdale PA
Jenson Qi - Branchburg NJ

Ortho-McNeil Pharmaceutical, Inc. - Raritan NJ

C12N 1562

435 697, 435 23, 435226, 4352523, 4353201, 536 232, 536 234

Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed protease C-E. The deduced amino acid sequence, and it alignment with other well-characterized serine proteases indicates that it is a member of the S1 serine protease family. We have found that the protease C-E mRNA is expressed in pancreas, placenta, prostate, small intestine, stomach, spleen, fibroblasts and epidermis, as well as in certain regions of the brain i. e. , cerebellum, cerebral cortex, pituitary and hippocampus. Enzymatically active protease C-E, as produced using the methodologies described herein, is amenable to further biochemical analyses for the identification of physiological substrates and specific modulators.

6458564, Oct 1, 2002

Aug 31, 1999

09/386653

Andrew Darrow - Lansdale PA
Jenson Qi - Branchburg NJ

Ortho-McNeil Pharmaceutical, Inc. - Raritan NJ

C12N 1562

435 697, 435 23, 435 691, 435226, 4352523, 4353201, 514 2, 536 232, 536 234

Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed protease T. The deduced amino acid sequence encodes a prepro form of 290 amino acids, and its alignment with other well-characterized serine proteases indicates that it is a member of the S1 serine protease family. We have found that the protease T mRNA is expressed in stomach, testis, retina, fibroblasts, spinal cord, and several regions of the brain. Protease T MRNA is also found in leukocytes and in the Jurkat (ATCC TIB-152) T cell line. Thus, this protease is potentially involved in gastric, testicular, retinal, dematological, neurological/neurodegenerative and/or immunological disorders. The protease T gene maps to human chromosome 16p13. 3 which is near the tryptase locus.

6485957, Nov 26, 2002

Aug 31, 1999

09/387375

Andrew Darrow - Lansdale PA
Jenson Qi - Branchburg NJ
Patricia Andrade-Gordon - Doylestown PA

Ortho-McNeil Pharmaceutical, Inc. - Raritan NJ

C12N 964

435226, 435 691, 435 697, 4352523, 4353201, 536 232, 536 234, 514 2

Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed protease EOS. The deduced amino acid sequence, and it alignment with other well-characterized serine proteases indicates that it is a member of the S1 serine protease family. We have found that the protease EOS mRNA is expressed in platelets and leukocytes and more specifically eosinophils. Although this protease is abundantly expressed in ovary, retina and stomach, where it may perform important functions, its expression in platelets and certain cells of the immune system suggests that it may play roles in thrombosis and in the immune process. Enzymatically active protease EOS is amenable to further biochemical analyses for the identification of physiological substrates and specific modulators.

6747134, Jun 8, 2004

Jan 8, 2002

10/041400

Andrew Darrow - Lansdale PA
Jenson Qi - Branchburg NJ
Patricia Andrade-Gordon - Doylestown PA

Ortho-McNeil Pharmaceutical, Inc. - Raritan NJ

C07K 1608

53038826, 5303891

Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed protease EOS. The deduced amino acid sequence, and it alignment with other well-characterized serine proteases indicates that it is a member of the S1 serine protease family. We have found that the protease EOS mRNA is expressed in platelets and leukocytes and more specifically eosinophils. Although this protease is abundantly expressed in ovary, retina and stomach, where it may perform important functions, its expression in platelets and certain cells of the immune system suggests that it may play roles in thrombosis and in the immune process. Enzymatically active protease EOS is amenable to further biochemical analyses for the identification of physiological substrates and specific modulators.

6750034, Jun 15, 2004

Jun 30, 2000

09/607745

Andrew L. Darrow - Lansdale PA
Jenson (Jian-Shen) Qi - Branchburg NJ
Patricia Andrade-Gordon - Doylestown PA

Ortho-McNeil Pharmaceutical, Inc. - Raritan NJ

C12Q 134

435 23, 435226, 435 18, 436546, 436173

Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed D-G. The deduced amino acid sequence, and its alignment with other well characterized serine proteases clearly indicates that it is a member of the S1 serine protease family. We have found that the protease D-G mRNA is widely expressed in several tissues throughout the body including epidermis, fibroblasts, keratinocytes, colon, small intestine, stomach, lung, kidney, bone marrow, lymph node, thymus, ovary, prostate, uterus and spinal cord. Interestingly, this protease contains a hydrophobic stretch of amino acids which is a putative transmembrane near the NH -terminus. Thus, this serine protease is thought to be synthesized as a type II integral protein. We expressed a soluble form of this novel human protease by inserting the portion of the protease D-G cDNA, encoding the catalytic domain, in a zymogen activation construct designed to permit the generic activation of heterologous serine protease catalytic domains. The result is an active preparation of protease D-G that has an activity against a subset of amidolytic substrates.

6806059, Oct 19, 2004

Jan 8, 2002

10/042091

Andrew Darrow - Lansdale PA
Jenson Qi - Branchburg NJ
Patricia Andrade-Gordon - Doylestown PA

Orth-McNeil Pharmaceutical, Inc. - Raritan NJ

C12Q 137

435 23, 435226

Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed protease EOS. The deduced amino acid sequence, and it alignment with other well-characterized serine proteases indicates that it is a member of the S1 serine protease family. We have found that the protease EOS mRNA is expressed in platelets and leukocytes and more specifically eosinophils. Although this protease is abundantly expressed in ovary, retina and stomach, where it may perform important functions, its expression in platelets and certain cells of the immune system suggests that it may play roles in thrombosis and in the immune process. Enzymatically active protease EOS is amenable to further biochemical analyses for the identification of physiological substrates and specific modulators.

6846920, Jan 25, 2005

Jan 8, 2002

10/041264

Andrew Darrow - Lansdale PA, US
Jenson Qi - Branchburg NJ, US
Patricia Andrade-Gordon - Doylestown PA, US

Ortho-McNeil Pharmaceutical, Inc. - Raritan NJ

C12N 1552
C12N 1562
C12N 964
C12N 1579
C12N 1585

536 232, 536 234, 435 691, 435 697, 4352523, 4353201, 435975

Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed protease EOS. The deduced amino acid sequence, and it alignment with other well-characterized serine proteases indicates that it is a member of the S1 serine protease family. We have found that the protease EOS mRNA is expressed in platelets and leukocytes and more specifically eosinophils. Although this protease is abundantly expressed in ovary, retina and stomach, where it may perform important functions, its expression in platelets and certain cells of the immune system suggests that it may play roles in thrombosis and in the immune process. Enzymatically active protease EOS is amenable to further biochemical analyses for the identification of physiological substrates and specific modulators.