Bernardo M Costa, Age 48840 C St UNIT 113, San Rafael, CA 94901

2008029, Nov 27, 2008

Apr 23, 2008

12/108278

Andreas Schirmer - Fremont CA, US
Zhihao Hu - Castro Valley CA, US
Bernardo da Costa - San Francisco CA, US

LS9, Inc. - South San Francisco CA

C12Q 1/68
C12N 1/21

435 6, 43525233, 4352523

Disclosed is a method using an alkane response element (ARE) from, e.g., spp. to (i) identify and clone hydrocarbon biosynthesis genes, (ii) identify and clone hydrocarbon transporter genes (iii) identify and clone hydrocarbon response genes. Screening cells were developed that expressed a transcriptional activator, e.g., alkR, and included a reporter gene, e.g., GFP operatively linked to an ARE promoter, e.g., the alkM promoter. The cells were transformed with libraries from organisms capable of hydrocarbon biosynthesis. Transformed cells that expressed the reporter gene harbored library-derived genes involved in one or more of the above-mentioned processes; and these genes were isolated from the cells using standard molecular biology techniques. Additional systems were designed wherein screening cells also expressed a gene identified in the original screen, e.g., an additional hydrocarbon pathway gene, e.g., an enhancer.

2019037, Dec 12, 2019

Nov 14, 2018

16/191096

- Ames IA, US
Bernardo Da Costa - South San Francisco CA, US

C12P 5/02
C12Q 1/02
C12P 7/26
C12N 9/00
C12N 9/88
C12N 9/16
C10L 1/04
C12P 7/64
C12N 9/10
C12N 9/02
C10L 1/08
C10L 1/06
C10G 3/00

The invention provides isolated nucleic acids and isolated polypeptides involved in the synthesis of hydrocarbons and hydrocarbon intermediates. Homologs of, conservative variants of, and sequences having at least about 35% sequence identity with nucleic acids involved in the synthesis of hydrocarbons and hydrocarbon intermediates are also provided. The invention further provides methods for producing an aliphatic ketone or a hydrocarbon, as well as a method for identifying an enzyme useful for the production of hydrocarbons.

2016007, Mar 17, 2016

Nov 25, 2015

14/952720

- Ames IA, US
Bernardo da Costa - South San Francisco CA, US

C12P 5/02
C12Q 1/02
C10L 1/04
C12N 9/16
C12N 9/88
C12P 7/26
C12N 9/00

The invention provides isolated nucleic acids and isolated polypeptides involved in the synthesis of hydrocarbons and hydrocarbon intermediates. Homologs of, conservative variants of, and sequences having at least about 35% sequence identity with nucleic acids involved in the synthesis of hydrocarbons and hydrocarbon intermediates are also provided. The invention further provides methods for producing an aliphatic ketone or a hydrocarbon, as well as a method for identifying an enzyme useful for the production of hydrocarbons.

2016000, Jan 7, 2016

Dec 11, 2013

14/760204

- South San Francisco CA, US
Bernardo DA COSTA - South San Francisco CA, US
Mathew RUDE - South San Francisco CA, US
Na TRINH - South San Francisco CA, US
Emanuela POPOVA - South San Francisco CA, US
Sankaranarayanan VENKITESWARAN - South San Francisco CA, US
Noah HELMAN - South San Francisco CA, US
Kevin HOLDEN - South San Francisco CA, US

REG LIFE SCIENCES, LLC - South San Francisco CA

C12P 7/64
C12N 9/16
C12N 9/12
C12P 7/04
C12N 9/02

The disclosure relates to recombinant microorganisms that exhibit an increased expression of an acyl carrier protein (ACP) resulting in production of fatty acid derivatives. The disclosure further relates to methods of using the recombinant microorganisms in fermentation cultures in order to produce fatty acid derivatives and related compositions.