Daniel Archie Mclaurin, Age 754509 Irene Way, Raleigh, NC 27603

4812293, Mar 14, 1989

Feb 22, 1988

7/159485

Daniel A. McLaurin - Cary NC
Beverly M. Fleming - Raleigh NC
James P. O'Connell - Chapel Hill NC

Becton, Dickinson and Company - Franklin Lakes NJ

G01N 118
B01L 300

422 69

A method for assay of an analyte and assay device therefor. The assay device includes an assay component affixed to a permeable membrane. The membrane preferably rests on a permeable membrane support and covers an opening through a receptacle for holding a liquid. The support is adjacent the open upper end of a double ended needle, the lower end of which is adapted to puncture a plug inserted in an open end of a preevacuated container. Puncturing of the plug establishes fluid communication between the prevacuated interior of the container and the opening in the receptacle whereby liquid in the receptacle is passed through the membrane. The membrane has a bubble point sufficient to prevent passage of air through the membrane after all of the liquid has been pulled through as long as the membrane remains wet so that vacuum in the tube is not lost. The device may be part of an assay workstation which comprises a canister and various utensils useful in performing the assay.

5208143, May 4, 1993

Sep 30, 1991

7/767351

Glenn L. Henderson - Durham NC
Randal A. Hoke - Cary NC
Anne C. Hopkins - Cary NC
Daniel A. McLaurin - Cary NC

Becton, Dickinson and Company - Franklin Lakes NJ

C12Q 170
C12Q 100

435 5

A method for immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate to a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.

6235464, May 22, 2001

Oct 20, 1994

8/326304

Glenn L. Henderson - Raleigh NC
Randal A. Hoke - Cary NC
Anne C. Hopkins - Cary NC
Daniel A. McLaurin - Cary NC

C12Q 170

435 5

A method for immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate to a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.