David A Gelfand, Age 66Richmond, CA

6346379, Feb 12, 2002

Sep 3, 1998

09/146631

David Harrow Gelfand - Oakland CA
Lisa Vivian Kalman - San Francisco CA
Fred Lawrence Reichert - Oakland CA
Christopher Lim Sigua - Antioch CA
Thomas W. Myers - Alameda CA

F. Hoffman-La Roche AG - Basel

C12Q 168

435 6, 435 911, 530300, 530327, 536 231, 536 232

Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.

6514736, Feb 4, 2003

Nov 1, 2000

09/704357

Henry A. Erlich - Oakland CA
Glenn Horn - Emeryville CA
Randall K. Saiki - Richmond CA
Kary B. Mullis - La Jolla CA
David H. Gelfand - Oakland CA

Roche Molecular Systems, Inc - Alameda CA

C12N 912

435194, 435 6, 435 912, 536 231, 536 243

The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

7141377, Nov 28, 2006

Jan 27, 2005

11/043099

David H. Gelfand - Oakland CA, US
Pamela M. Holland - Seattle WA, US
Randall K. Saiki - Richmond CA, US
Robert M. Watson - Berkeley CA, US

Roche Molecular Systems, Inc. - Alameda CA

C12Q 1/68
C12P 19/34

435 6, 435 911, 435 912, 435810, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, 935 88

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.

7148049, Dec 12, 2006

Mar 26, 2003

10/401403

Nancy J. Schoenbrunner - Moraga CA, US
Thomas W. Myers - Alameda CA, US
David H. Gelfand - Oakland CA, US

Roche Molecular Systems, Inc. - Alameda CA

C12N 9/12

435194, 435196

The present invention provides thermostable or thermoactive DNA polymerases with attenuated 3′-5′ exonuclease activity, methods for their synthesis, methods for their use, kits comprising the polymerases, nucleic acids encoding the polymerases and cells comprising such a nucleic acid. The DNA polymerases of the invention are useful in many recombinant DNA techniques, such as nucleic acid amplification by the polymerase chain reaction. The DNA polymerases of the invention allow higher fidelity replication and amplification of a template DNA sequence, allow less degradation of primers and/or more efficient use of deoxynucleotide triphosphates and are in general more efficient and less costly to make and use.

7179590, Feb 20, 2007

Mar 30, 2001

09/823649

Edward Soh Smith - San Francisco CA, US
Carita Maria Elfstrom - San Francisco CA, US
David Harrow Gelfand - Oakland CA, US
Russell Gene Higuchi - Alameda CA, US
Thomas William Myers - Alameda CA, US
Alice Ming Wang - Lafayette CA, US

Roche Molecular Systems, Inc - Alameda CA

C07H 21/04
C12Q 1/68

435 6, 435 911, 435 912, 435 9121, 536 231

The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.

7445900, Nov 4, 2008

Aug 11, 2005

11/203461

David H. Gelfand - Oakland CA, US
Pamela M. Holland - Seattle WA, US
Randall K. Saiki - Richmond CA, US
Robert M. Watson - Berkeley CA, US

Roche Molecular Systems, Inc. - Pleasanton CA

C12Q 1/68
C12P 19/34

435 6, 435 912

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.

7572581, Aug 11, 2009

Jun 28, 2004

10/879493

David Harrow Gelfand - Oakland CA, US
Fred Lawrence Reichert - San Leandro CA, US
Veeraiah Bodepudi - San Ramon CA, US
Amar Gupta - Danville CA, US
Stephen Will - Oakland CA, US
Thomas Myers - Alameda CA, US

Roche Molecular Systems, Inc. - Pleasanton CA

C12Q 1/68
C07H 19/00

435 6, 435975, 536 221

The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.

7598032, Oct 6, 2009

Jan 30, 2003

10/355532

David Harrow Gelfand - Oakland CA, US
Lisa Vivian Kalman - San Francisco CA, US
Fred Lawrence Reichert - Oakland CA, US
Christopher Lim Sigua - Antioch CA, US
Thomas W. Myers - Alameda CA, US

Roche Molecular Systems - Pleasanton CA

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04

435 6, 435 912

Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.