Dawn Renee Applegate, Age 614468 Saratoga Ave, San Diego, CA 92107

2011029, Dec 1, 2011

Oct 22, 2009

13/125798

Dawn R. Applegate - San Diego CA, US

RegeneMed, Inc. - San Diego CA

C12N 5/071
C12M 3/00

435401, 4353031, 4353052

A microplate is provided herein having a plate body with at least one well formed therein, the well having a first open end, a second end, an aperture being formed in the second end, and a side wall extending between the first end and the second end. The microplate further has a permeable membrane extending at least partially across the aperture formed in the second end. A microplate is provided with a permeable membrane which allows not only for removal of certain solutes from a solution (high or low molecular weight solutes), but also allows for separation of macromolecular mixtures and degradation of the membrane. The microplate is also provided with an integrated top assembly or integrated inserts. Also provided herein are methods of culturing systems in the microplates described.

2013026, Oct 3, 2013

May 30, 2013

13/905510

Dawn R. Applegate - San Diego CA, US

REGENEMED, INC. - San Diego CA

C12N 5/00

4353052

A microplate is provided herein having a plate body with at least one well formed therein, the well having a first open end, a second end, an aperture being formed in the second end, and a side wall extending between the first end and the second end. The microplate further has a permeable membrane extending at least partially across the aperture formed in the second end. A microplate is provided with a permeable membrane which allows not only for removal of certain solutes from a solution (high or low molecular weight solutes), but also allows for separation of macromolecular mixtures and degradation of the membrane. The microplate is also provided with an integrated top assembly or integrated inserts. Also provided herein are methods of culturing systems in the microplates described.

2013026, Oct 3, 2013

May 30, 2013

13/905487

Dawn R. Applegate - San Diego CA, US

REGENEMED, INC. - San Diego CA

C12N 5/00

435401

A microplate is provided herein having a plate body with at least one well formed therein, the well having a first open end, a second end, an aperture being formed in the second end, and a side wall extending between the first end and the second end. The microplate further has a permeable membrane extending at least partially across the aperture formed in the second end. A microplate is provided with a permeable membrane which allows not only for removal of certain solutes from a solution (high or low molecular weight solutes), but also allows for separation of macromolecular mixtures and degradation of the membrane. The microplate is also provided with an integrated top assembly or integrated inserts. Also provided herein are methods of culturing systems in the microplates described.

5763267, Jun 9, 1998

Apr 16, 1996

8/632972

Christine Kurjan - Palo Alto CA
Mark A. Applegate - San Diego CA
James H. Flatt - Del Mar CA
Dawn Orton Applegate - San Diego CA
Nicole Bloom - San Francisco CA
Mark Baumgartner - San Diego CA

Advanced Tissue Sciences - La Jolla CA

C12M 132
C12M 122
C12C 100

4352931

An apparatus for the large scale culturing and packaging of cell suspensions, three dimensional tissue, and other biological systems is disclosed. The apparatus includes a plurality of flexible or semi-flexible treatment chambers comprising one or more individual culture pockets, a plurality of rigid spacers, an inlet fluid manifold, an outlet fluid manifold, a fluid reservoir, and a means for transporting fluid within the system. During treatment, liquid media is transported from the fluid reservoir to the inlet manifold, which will in turn evenly distribute the media to each of the connected treatment chambers and internal culture pockets. An outlet fluid manifold is also provided to ensure that each treatment chamber is evenly filled and to ensure that any air bubbles formed during treatment are removed from the treatment chambers. The treatment chambers are flexible or semi-flexible so as to provide for easy end-user handling during rinsing and application of the cultured transplants. Due to the flexibility of the treatment chambers, rigid spacers are also provided which ensure even fluid distribution within the chambers during treatment.

5843766, Dec 1, 1998

Feb 5, 1996

8/597053

Dawn Orton Applegate - San Diego CA
Mark Applegate - San Diego CA
Mark Baumgartner - San Diego CA
John W. Bennett - San Diego CA
John Danssaert - San Diego CA
Robert Hardin - San Diego CA
Lee Laiterman - Los Gatos CA
Fred Schramm - Del Mar CA
William R. Tolbert - San Diego CA

Advanced Tissue Sciences, Inc. - La Jolla CA

C12M 300

4352841

A tissue culture chamber is disclosed. The chamber is a casing that provides for growth of three-dimensional tissue, and more specifically for the growth of skin tissue, that can be grown, preserved in frozen form, and shipped to the end user in the same aseptic container. The tissue culture chamber includes a casing comprising a substrate within the casing designed to facilitate three-dimensional tissue growth on the surface of the substrate. The casing includes an inlet and an outlet port which assist the inflow and outflow of media. The casing also includes at least one flow distributor. In one embodiment, the flow distributor is a baffle, which is used to distribute the flow of the media within the chamber to create a continuous, uniform piece of three-dimensional tissue. In a second embodiment, the flow distributor is a combination of deflector plates, distribution channels, and a flow channel. In each embodiment, the casing further includes a seal so as to ensure an aseptic environment inside the chamber during tissue growth and storage.