Erik G Meyer, Age 441418 Fitzwatertown Rd, Willow Grove, PA 19090

2008013, Jun 12, 2008

Oct 10, 2007

11/974016

Samuil R. Umansky - Princeton NJ, US
Hovsep Melkonyan - Princeton NJ, US
Erik Meyer - Princeton NJ, US
William John Feaver - East Brunswick NJ, US

C07H 21/02
C07H 21/04

536 2541

This invention provides compositions and methods for rapid separation, isolation and purification of nucleopolymers, especially nucleic acids, from biological samples using anionic exchange media. The method of the present invention can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution. The medium/nucleoprotein bound complex may be optionally stored or transported, but is subsequently treated with appropriate eluents to remove any undesirable proteins, including destabilizing enzymes, and inorganic salts and the like. The partially purified complex may also be stored or transported prior to further processing. The instant method is particularly advantageous as it provides the means for rapid isolation and purification of soluble nucleic acids obtained from samples of biological fluids of comparatively large volume, allowing ease of handling and storage, and stabilizing the samples against degradation prior to analysis. It also permits the purification and identification of shorter fragments of nucleic acids from bodily fluids which, up till now, had not been identified. The method is widely useful as a means to prepare samples formats that are convenient in diagnostic analytical methods, particularly those that rely on the detection, characterization and identification of nucleic acid sequences, from body fluids.

2015027, Oct 1, 2015

Apr 30, 2015

14/701265

- San Diego CA, US
Hovsep S Melkonyan - Princeton NJ, US
Erik Meyer - Princeton NJ, US
William John Feaver - E Brunswick NJ, US

TROVAGENE, INC. - San Diego CA

C12N 15/10
C12Q 1/68

This invention provides compositions and methods for rapid separation, isolation and purification of nucleic acids from biological samples using anionic exchange media. The method can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution. The instant method is particularly advantageous as it permits the purification and identification of shorter fragments of nucleic acids from bodily fluids which, until now, had not been identified.