Bo Wen Huang, Age 5012 Lincoln Rd, Sharon, MA 02067

8564792, Oct 22, 2013

Dec 19, 2008

12/746784

Xiaowei Zhuang - Cambridge MA, US
Bo Huang - San Francisco CA, US
Wilfred M. Bates - Goettingen, DE
Wenqin Wang - Somerville MA, US

President and Fellows of Harvard College - Cambridge MA

G01B 11/14

356624, 356614

The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques, including imaging in three dimensions. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. In some cases, the position of the entities can be determined in all three spatial dimensions (i. e. , in the x, y, and z directions), and in certain cases, the positions in all three dimensions can be determined to an accuracy of less than about 1000 nm. In some cases, the z positions may be determined using one of a variety of techniques that uses intensity information or focal information (e. g. , a lack of focus) to determine the z position. Non-limiting examples of such techniques include astigmatism imaging, off-focus imaging, or multi-focal-plane imaging.

2008018, Jul 31, 2008

Feb 1, 2008

12/012524

Xiaowei Zhuang - Cambridge MA, US
Wilfred M. Bates - Somerville MA, US
Michael J. Rust - Medford MA, US
Bo Huang - Somerville MA, US

President and Fellows of Harvard College - Cambridge MA

G01N 21/76

436172

The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g., by determining light emitted by the entity), then a second entity may be activated and determined. The entities may be immobilized relative to each other and/or to a common entity. The emitted light may be used to determine the positions of the first and second entities, for example, using Gaussian fitting or other mathematical techniques, and in some cases, with sub-diffraction limit resolution. The methods may thus be used, for example, to determine the locations of two or more entities immobilized relative to a common entity, for example, a surface, or a biological entity such as DNA, a protein, a cell, a tissue, etc. The entities may also be determined with respect to time, for example, to determine a time-varying reaction. Other aspects of the invention relate to systems for sub-diffraction limit image resolution, computer programs and techniques for sub-diffraction limit image resolution, methods for promoting sub-diffraction limit image resolution, methods for producing photoswitchable entities, and the like.

2010029, Nov 25, 2010

Aug 4, 2010

12/850586

Xiaowei Zhuang - Cambridge MA, US
Wilfred M. Bates - Somerville MA, US
Michael J. Rust - Medford MA, US
Bo Huang - San Francisco CA, US
Graham Thomas Dempsey - Somerville MA, US

President and Fellows of Harvard College - Cambridge MA

G01N 21/64
C07K 2/00
C07K 16/00

436172, 530402, 5303913

The present invention generally relates to sub-diffraction limit image resolution and other imaging techniques. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g., by determining light emitted by the entity), then a second entity may be activated and determined The entities may be immobilized relative to each other and/or to a common entity. The emitted light may be used to determine the positions of the first and second entities, for example, using Gaussian fitting or other mathematical techniques, and in some cases, with sub-diffraction limit resolution. The methods may thus be used, for example, to determine the locations of two or more entities immobilized relative to a common entity, for example, a surface, or a biological entity such as DNA, a protein, a cell, a tissue, etc. The entities may also be determined with respect to time, for example, to determine a time-varying reaction. Other aspects of the invention relate to systems for sub-diffraction limit image resolution, computer programs and techniques for sub-diffraction limit image resolution, methods for promoting sub-diffraction limit image resolution, methods for producing photoswitchable entities, and the like.

2017000, Jan 5, 2017

Sep 19, 2016

15/268696

- El Segundo CA, US
Bo HUANG - Redondo Beach CA, US
Heather N. TRUONG - Anaheim CA, US
Ruben D. Lechuga - Palos Verdes Peninsula CA, US
Joshua N. Snow - Los Angeles CA, US
Michael E. Depies - Lawndale CA, US
Roshan Menon - San Pedro CA, US
Neil McIver - Llandudno, GB

The DIRECTV Group, Inc. - El Segundo CA

H04N 21/482
H04N 21/81
H04N 21/435
H04N 21/431
H04N 21/433

A user device for storing or displaying content recommendations includes a search session manager receiving metadata associated with content. The metadata includes a received hierarchical identifier including a primary level and a secondary level unique to the primary level. A memory stores selected hierarchical identifiers. A search module compares the received hierarchical identifiers with the selected hierarchical identifiers. A screen display displays content from at least one of the primary level and secondary level based on comparing.

2015028, Oct 8, 2015

Apr 7, 2014

14/246835

- El Segundo CA, US
Earl J. Bonovich - Tinley Park CA, US
Luke J. Crook - Torrance CA, US
Timothy W. Frech - Huntington Beach CA, US
Bo Huang - Redondo Beach CA, US
Eileen Loh - Rowland Heights CA, US

THE DIRECTV GROUP, INC. - El Segundo CA

G06F 3/0484
G06F 3/0488

A system and method for moving displayed content between a first device and a second device includes a first display displaying video content having a first content identifier. A first device forms a transfer signal by a gesture or a user interface. The first device communicates a query command signal comprising the first content identifier to a separate web service. The first device receives an asset data signal comprising at least a second content identifier from the web service in response to the query command signal. The second content identifier relates to the first content identifier. One of the first device and the second device requests the video content based on the second identifier and a resume point. The resume point corresponds to a playback position of the video content being displayed at about a time of generating the transfer signal and a second display displays the video content.

2015005, Feb 19, 2015

Aug 7, 2014

14/454653

- El Segundo CA, US
Bo Huang - Redondo Beach CA, US
Heather N. Truong - Anaheim CA, US
Ruben D. Lechuga - Palos Verdes Peninsula CA, US
Joshua N. Snow - Los Angeles CA, US
Michael E. Depies - Lawndale CA, US
Roshan Menon - San Pedro CA, US
Neil McIver - Llandudno, GB

THE DIRECTV GROUP, INC. - El Segundo CA

H04N 21/431
H04N 21/482
H04N 21/81
G06F 17/30
G06F 3/0482

725 44

A user device for storing or displaying content recommendations includes a search session manager receiving metadata associated with content. The metadata includes a received hierarchical identifier including a primary level and a secondary level unique to the primary level. A memory stores selected hierarchical identifiers. A search module compares the received hierarchical identifiers with the selected hierarchical identifiers. A screen display displays content from at least one of the primary level and secondary level based on comparing.