Inventors:
- Santa Clara CA, US
Corissa Harris - Santa Clara CA, US
Matt Dipetro - Gilroy CA, US
Mark Ambroso - San Diego CA, US
Yaozhong Zou - Sunnyvale CA, US
Marshall Porter - Petaluma CA, US
Issa Delkaninia - San Jose CA, US
Alexxa Noble - San Jose CA, US
James Fairman - Sunnyvale CA, US
Wiseley Wu - Newark CA, US
Kapil Bajaj - Newark CA, US
Giovanni Bellesia - San Jose CA, US
Seong-Ho Shin - Oakland CA, US
Assignee:
GENIA TECHNOLOGIES, INC. - Santa Clara CA
International Classification:
G01N 33/487
C07K 14/31
Abstract:
Described herein are nanopore protein conjugates that can be used in DNA sequencing reactions. The nanopore protein conjugates includes a nanopore protein monomer that is joined to a DNA binding domain. The nanopore protein monomer is available to oligomerize with other nanopore protein monomers, while the DNA binding domain is available to bind to a template DNA strand. In certain examples, the nanopore protein monomer is an alpha-hemolysin monomer or variant thereof and the DNA binding domain is an Sso7d protein or variant thereof, such as an Sso7d-like protein. Also provided are nanopore protein assemblies incorporating the nanopore protein conjugates, along with methods of using the nanopore protein assemblies in sequencing reactions.
