James L Mohler, Age 7123 Lincoln Woods Ln, Buffalo, NY 14222

8241845, Aug 14, 2012

Mar 16, 2009

12/404590

James L. Mohler - Buffalo NY, US
Mark Titus - Chapel Hill NC, US
Olga Kozyreva - Chapel Hill NC, US
Elsbieta Kawinski - Orchard Park NY, US
Yun Li - Orchard Park NY, US

Health Research Inc. - Buffalo NY

C12Q 1/68
C12Q 1/26
C12N 15/53
C12N 9/02

435 6, 435 25, 435189

The invention provides an isolated, novel steroid 5α-reductase enzyme termed SRD5AIII. The protein has an estimated molecular weight of 37 kDa and is capable of converting testosterone to dihydrotestosterone at a pH of about 7. Also provided is a method for identifying inhibitors of SRD5AIII by contacting SRD5AIII with a test compound and measuring the activity of the enzyme. A reduced activity relative to a control indicates that the test compound is an inhibitor of SRD5AIII. A method is also provided for detecting androgen stimulated prostate cancer or recurrent prostate cancer in an individual. The method comprises obtaining a prostate biopsy from an individual and determining the level of expression of SRD5AIII gene or protein relative to a normal control. An increased expression of SRD5AIII relative to the control is indicative of androgen stimulated prostate cancer or recurrent prostate cancer.

2006027, Dec 7, 2006

May 10, 2006

11/432424

James Mohler - Buffalo NY, US
Mark Titus - Chapel Hill NC, US
Olga Kozyreva - Chapel Hill NC, US
O. Ford - Wake Forest NC, US
Elsbieta Kawinski - Orchard Park NY, US
Yun Li - Orchard Park NY, US

C12Q 1/68
G01N 33/574
C12N 9/02
C12N 15/861

435006000, 435007230, 435189000, 435456000, 435183000

The invention provides an isolated, novel steroid 5α-reductase enzyme termed SRD5AIII. The protein has an estimated molecular weight of 37 kDa and is capable of converting testosterone to dihydrotestosterone at a pH of about 7.0. Also provided is a method for identifying inhibitors of SRD5AIII by contacting SRD5AIII with a test compound and measuring the activity of the enzyme. A reduced activity relative to a control indicates that the test compound is an inhibitor of SRD5AIII. A method is also provided for detecting androgen stimulated prostate cancer or recurrent prostate cancer in an individual. The method comprises obtaining a prostate biopsy from an individual and determining the level of expression of SRD5AIII gene or protein relative to a normal control. An increased expression of SRD5AIII relative to the control is indicative of androgen stimulated prostate cancer or recurrent prostate cancer.

2008017, Jul 24, 2008

Oct 8, 2007

11/973127

James L. Mohler - Buffalo NY, US
Mark Titus - East Amherst NY, US
Kenneth Tomer - Cary NC, US

C12Q 1/02
G01N 33/92

435 29, 436 71

Provided is a method for determining the presence or amount of 5-α-dihydro-3-keto steroids (ADKSs) comprising the steps of preparing a sample for chromatographic separation, subjecting the sample to chromatographic separation, subjecting the chromatographically separated sample to atmospheric pressure photoionization (APPI), and detecting the amount of alkylated parent ions using mass spectrometry or methylated daughter ions by mass spectrometry after collisionally induced dissociation of the alkylated parent ions. Tandem mass spectrometry of ADKSs with a 6-member A ring and a 3-keto group using APPI and methanol as the alkoxy donor will produce methylated daughter ions with a mass/charge ratio of 85.0. Quantitation of this alkylated daughter ion provides a method to detect very low levels of ADKSs such as DHT. This method can also be used for the simultaneous detection of ADKSs and non-ADKSs.

2020028, Sep 10, 2020

Oct 26, 2018

16/758139

- Buffalo NY, US
James L. MOHLER - Buffalo NY, US

A61F 13/471
A61F 13/62
A61F 13/78
A61F 13/56

The present disclosure may be embodied as an incontinence device for a catheterized individual. The device includes an absorbent pad configured to be worn by the individual. The absorbent pad having a slit partially separating the pad into two portions. A fastener may be provided for selectively attaching the two portions to each other. In this way, a catheter may be disposed through the slit of the device and the two portions may be attached to each other using the fastener to surround a circumference of the catheter. The fastener may be reusable. In some embodiments, the device includes more than one fastener. The fastener may be one or more of an adhesive, a hook-and-loop fastener, a snap fastener, or combinations thereof.