Jason A Wilken, Age 471134 Post St, Alameda, CA 94501

Jason Wilken Phones & Addresses

Alameda, CA

428 Alice St APT 822, Oakland, CA 94607

104 Edwards St, New Haven, CT 06511

305 Saint John St, New Haven, CT 06511 (203) 787-6167

Omaha, NE

5010 Charles St, Omaha, NE 68132

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Us Patents

Method For Purifying A Protein Of The Cystine-Knot Superfamily

US Patent:
2006000, Jan 12, 2006
Filed:
Jul 8, 2004
Appl. No.:
10/887106
Inventors:
Elliott Bedows - Bellevue NE, US
Jason Wilken - Omaha NE, US
International Classification:
C07K 1/10
C07K 14/705
US Classification:
530412000
Abstract:
The present invention relates to a two-step method for isolating proteins from the cystine-knot superfamily based on dye ligand affinity chromatography and reversed-phase chromatography. Advantageously, the method can be performed in a relatively short period of time, involves inexpensive reagents, and requires little sample preparation before and during the purification process. Protein fusions between cystine-knot proteins and proteins of interest are further provided for the isolation of said protein of interest or complexes containing said protein of interest using the two-step method disclosed.

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