John A Browse, Age 7241 Mckenzie Rd, Palouse, WA 99161

John Browse Phones & Addresses

41 Mckenzie Rd, Palouse, WA 99161 (509) 878-1251

Pullman, WA

41 Mckenzie Rd, Palouse, WA 99161

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Us Patents

Desaturases And Methods Of Using Them For Synthesis Of Polyunsaturated Fatty Acids

US Patent:
6825017, Nov 30, 2004
Filed:
Aug 17, 2001
Appl. No.:
09/857583
Inventors:
John A. Browse - Palouse WA
James G. Wallis - Moscow ID
Jennifer L. Watts - Moscow ID
Assignee:
Washington State University Research Foundation - Pullman WA
International Classification:
C12P 2106
US Classification:
435190, 435 4, 435 6, 435 691, 435183, 43525234, 4353201, 435325, 536 232, 536 237
Abstract:
The amino acid and nucleic acid sequences of a -desaturase enzyme and a -desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.

Acyl Coenzyme A Thioesterases

US Patent:
6878861, Apr 12, 2005
Filed:
Jul 16, 2001
Appl. No.:
09/906408
Inventors:
Gregory B. Tilton - Pullman WA, US
Jay M. Shockey - Pullman WA, US
John A. Browse - Pullman WA, US
Assignee:
Washington State University Research Foundation - Pullman WA
International Classification:
C12N015/82
A01H005/00
US Classification:
800298, 800281, 435 6, 435 74, 536 232
Abstract:
The present invention provides compositions and methods related to acyl-coenzyme A thioesterases. In particular, the present invention is related to plant acyl-coenzyme A thioesterases. The present invention encompasses both native and recombinant wild-type forms of the enzymes, as well as mutant and variant forms, some of which possess altered characteristics relative to the wild-type enzyme. The present invention also relates to methods of using acyl-CoA thioesterases, including altered expression in transgenic plants and expression in prokaryotes and cell culture systems.

Ω-3 Fatty Acid Desaturase

US Patent:
6884921, Apr 26, 2005
Filed:
Mar 21, 2002
Appl. No.:
10/104339
Inventors:
John A. Browse - Palouse WA, US
James P. Spychalla - Antigo WI, US
Assignee:
Washington State University Research Foundation - Pullman WA
International Classification:
C12N015/82
C12P007/64
US Classification:
800281, 435 691, 435134
Abstract:
Recombinant expression of fat-1 gene of in a wide variety of cells, including cells of and , produces a polypeptide having ω-3 desaturase activity.

Plant Acyl-Coa Synthetases

US Patent:
7105722, Sep 12, 2006
Filed:
Apr 9, 2002
Appl. No.:
10/119136
Inventors:
Jay M. Shockey - Pullman WA, US
Judy Schnurr - Pullman WA, US
John A. Browse - Pullman WA, US
International Classification:
A01H 15/82
C12P 21/06
US Classification:
800281, 536 236, 435 691
Abstract:
The present invention relates to genes encoding plant acyl-CoA synthetases and methods of their use. In particular, the present invention is related to plant acyl-coenzyme A synthetases. The present invention encompasses both native and recombinant wild-type forms of the enzymes, as well as mutant and variant forms, some of which possess altered characteristics relative to the wild-type enzyme. The present invention also relates to methods of using acyl-CoA synthetases, including altered expression in transgenic plants and expression in prokaryotes and cell culture systems.

Desaturases And Methods Of Using Them For Synthesis Of Polyunsaturated Fatty Acids

US Patent:
7402735, Jul 22, 2008
Filed:
Oct 26, 2004
Appl. No.:
10/975692
Inventors:
John A. Browse - Palouse WA, US
James G. Wallis - Moscow ID, US
Jennifer L. Watts - Moscow ID, US
Assignee:
Washington State University Research Foundation - Pullman WA
International Classification:
A01H 5/00
US Classification:
800298
Abstract:
The amino acid and nucleic acid sequences of a Δ-desaturase enzyme and a Δ-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.

Enhancement Of Hydroxy Fatty Acid Accumulation In Oilseed Plants

US Patent:
8101818, Jan 24, 2012
Filed:
May 22, 2006
Appl. No.:
11/915146
Inventors:
John A. Browse - Palouse WA, US
Jay M. Shockey - Mandeville LA, US
Julie Jeannine Burgal - Davis CA, US
Assignee:
Washington State University - Pullman WA
International Classification:
A01H 1/00
A01H 5/00
A01H 5/10
A01H 15/00
C07H 21/04
C12N 5/14
C12N 15/82
US Classification:
800278, 800281, 800306, 800312, 800314, 8003201, 800322, 536 236, 435410, 4353201, 435468, 4352541
Abstract:
Particular aspects provide six novel cDNA clones, including cloned sequences of: DGAT (RcDGAT1 and RcDGAT2); RcLPAT; LACS (RcLACS4), and PDAT (RcPDAT1A and RcPDAT1B). Additional aspects provide methods for substantially enhanced accumulation of hydroxy fatty acid (HFA) in transgenic plant tissue (e. g. , seeds), comprising expression of particular novel sequences. For example, expression of RcDGAT2 or RcPDAT1 in castor hydroxylase-expressing lines resulted in substantially enhanced accumulation of hydroxy fatty acid (HFA) (e. g. , to over 30%; a 50-70% increase in HFA accumulation) relative to the hydroxylase-only expressing parental lines. Further aspects provide methods to increase at least one of total lipid content, percent seed germination, and seed weight in transgenic plants, comprising expression of RcDGAT2 in castor hydroxylase-expressing plant lines. Yet further aspects provide methods for expressing and accumulating hydroxyl fatty acid in yeast (e. g. , TAG biosynthesis from diricinolein), comprising expression of RcDGAT2 RcDGAT2 coding sequences in yeast.

Desaturases And Methods Of Using Them For Synthesis Of Polyunsaturated Fatty Acids

US Patent:
8124838, Feb 28, 2012
Filed:
Jun 18, 2008
Appl. No.:
12/141865
Inventors:
John A. Browse - Palouse WA, US
James G. Wallis - Moscow ID, US
Jennifer L. Watts - Moscow ID, US
Assignee:
Washington State University Research Foundation - Pullman WA
International Classification:
C12N 15/82
C12N 5/10
C12N 1/20
A01H 5/00
US Classification:
800281, 435419, 4352523
Abstract:
The amino acid and nucleic acid sequences of a Δ-desaturase enzyme and a Δ-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.

Omega-3 Fatty Acid Desaturase

US Patent:
2002004, Apr 11, 2002
Filed:
Dec 20, 2000
Appl. No.:
09/747755
Inventors:
John Browse - Palouse WA, US
James Spychalla - Antigo WI, US
International Classification:
A01H005/00
C12N015/82
US Classification:
800/298000, 435/419000, 435/468000
Abstract:
Recombinant expression of fat-1 gene of in a wide variety of cells, including cells of and , produces a polypeptide having -3 desaturase activity.

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