Kendall F Walker, Age 53110 Bigney Ave, Brockton, MA 02301

Greater Boston Area

Graphic Design

Massachusetts College of Art Sep 1991 - May 1994
BFA, Graphic Design Northern Arizona University 1989 - 1991
Associates, Graphic Design

United States

Tufts University School of Medicine May 2009 to 2000
Post-doctoral Fellow MGH - Boston, MA Apr 2008 to May 2009
Post-doctoral Fellow MGH - Boston, MA Oct 2006 to Apr 2008
Post-doctoral Fellow Murdoch University Aug 2003 to Oct 2006
Graduate Research Assistant Murdoch University 2000 to 2006
PhD candidate researcher; Advisors Sole Tradership 2002 to 2003
Sub-contractor Murdoch University 1999 to 1999
Honors candidate researcher; Advisors

Murdoch University 2006
PhD in Biology and Biomedical Science Murdoch University 1999
BSc. Hons in Biotechnology

In-vivo: Rodent surgery (Stereotaxic viral (AAV) and drug delivery (icv) and controlled cortical impact (CCI) model of TBI); Behavioral analysis (Motor - rotarod, beam-walk, wire-grip; Emotional - OFT, EPM, L/D transition, FST, marble burying and Cognitive - MWM, Y-maze, NOR & Fear-conditioning); Injection (i.p. and s.c.) of rodents; Handling, restraint, euthanasia, perfusion and dissection of large (ovine) and small (rodents) lab animals. Molecular Biology: Plasmid design and construction; Cloning; RNA/DNA extraction- blood and tissue; Standard, mutagenic and quantitative PCR; RACE; SSCP; Sequencing; Affymetrix microarray; Southern blotting (isotopic and chemi); Northern blotting (isotopic and chemi.); Nuclease protection assays. Protein: Recomb. protein expression (BEVS, HEK); Purification of recomb. and non-recomb. proteins (Affinity, ion-exchange, size-exclusion); Polyclonal antibody production and purification (rodents); Electrophoresis (native, denaturing, IEF, enzyme); Western blotting; Immunoprecipitation; ELISA (A, BACE1, p24); FRET based assays. Histology and Imaging: Tissue sectioning (Cryo. and Paraffin); Standard histological staining; Immunohistochemistry; Immunocytochemistry; Confocal microscopy. Cell Culture: Standard mammalian and non-mammalian cell culture (HEK, Cos-7, H4, Neuro2a, Sf9); transfection; siRNA and shRNA mediated gene silencing; Lentiviral packaging and infection; Primary cortical and hippocampal neuronal culturing and fibroblast culturing from embryonic and post-natal rodents. Software: Imaging- Metamorph, ImageJ, NIH Elements, MCID; Behavior- Ethovision XT, Motor Monitor, FreezeFrame

University of Massachusetts School for Marine Science & Technology 2009 to 2012
MS in Coastal Management Boston College 1997 to 2001
BS in Environmental Geoscience/minor Environmental Law and Policy

40-Hour OSHA Hazardous Waste Hazwoper Training, Institute for Environmental Edu. Inc. OSHA 1910.120 (e) (8) Hazardous Waste Hazwoper Refresher Training, Tetra Tech EC, Inc., April 2009 OSHA 1910.120 (e) (4) Hazwoper Supervisor Training, Tetra Tech FW, Inc. Occupational Safety and Health 10-hour Construction Training, OSHA Construction Quality Management for Contractors Training, USACE DOT/HM-126F Hazmat Training 49 CFR 172, Subpart H, Tetra Tech EC, Inc. Waste Management Employee Training 40 CFR 265.16, Tetra Tech EC, Inc. Project Management 100 Training, Tetra Tech FW, Inc. Project Management 201 Training, Tetra Tech EC, Inc. Construction Superintendent Training, Tetra Tech FW, Inc. NSC Defensive Driving Course 8th Edition, Tetra Tech EC, Inc.