Li I Liu817 Pinnacle Dr, Allentown, PA 18103

Management and Inspection Jul 1993 to Apr 2001
Manager in Sales Beijing Xiyuan Hotel Sep 1978 to 1993
Sales manager in Sales Department

Merck Research Laboratories/Schering-Plough Corp - Kenilworth, NJ Jan 2006 to Dec 2011
Scientist 1 Five Prime Therapeutics, Inc - South San Francisco, CA Jun 2003 to Jan 2006
Research Associate III, IV Sangamo Biosciences, Inc - Richmond, CA Jul 2002 to Apr 2003
Research Associate II Oklahoma Medical Research Foundation - Oklahoma City, OK Aug 2001 to Jun 2002
Research Assistant/Associate Department of Pharmacology & Toxicology, Indiana University School of Medicine - Indianapolis, IN Sep 1997 to Dec 1998
Research Technician Beijing Institute of Pharmacology and Toxicology Aug 1996 to Jul 1997
Technician

Molecular Biology: DNA and RNA isolation, PCR/RT-PCR and Taqman (real-time PCR), molecular cloning and Gateway cloning, generation of protein expression constructs, DNA sequencing. Cell Biology: Cell culture (including mouse embryonic stem cell), transfection. Transient expression of foreign genes in mammalian cells. Generation and maintenance of stable cell lines. Cell-based assay. Biochemistry: Cell lysate preparation, protein immunoprecipitation, SDS-PAGE, Western blotting, ELISA, protein purification by affinity chromatography, Meso Scale Discovery immunoassays. Animal Skills: Mice handling, genotyping, bleeding by cardiac puncture, subcutaneous and tail vein, dissection, tail vein injection and perfusion, tumor animal models. Isolation, microinjection and transfer of mouse blastocysts. Bile duct injection and islet isolation. Computer Skills: Microsoft word, Excel, PowerPoint, DNA Star, FileMaker, Prism, MSD software for data analysis.