Max D Summers, Age 86PO Box 224, Mesa, CO 81643

6017734, Jan 25, 2000

Jan 30, 1997

8/792832

Max D. Summers - Bryan TX
Sharon C. Braunagel - Bryan TX
Tao Hong - Bryan TX

The Texas A & M University System - College Station TX

C07H 2100
C12N 510
C12N 1533
C12N 1563

435 697

Provided are hydrophobic targeting sequences, which may serve to target heterologous proteins to a variety of cellular membranes. In particular, the structural components of the nuclear envelope, or those components which become nucleus-associated, may be targeted with the sequences provided. Also provided are methods of targeting heterologous proteins to particular membranes, and the use of these targeted proteins in therapeutic, diagnostic and insecticidal applications.

5023328, Jun 11, 1991

Aug 4, 1989

7/389377

Max D. Summers - Bryan TX
James Y. Bradfield - College Station TX
Larry L. Keeley - College Station TX

The Texas A&M University System - College Station TX

C12N 1516
C12N 1512

536 27

A baculovirus expression vector system is constructed wherein the natural signal DNA peptide sequence associated with the desired foreign gene, such as CD4 (T cell surface protein T4) is replaced by the signal DNA sequence from the Lepidopteran adipokinetic hormone (AKH) precursor signal peptides. The exemplary Manduca sexta AKH signal sequence is represented as follows: ##STR1##.

5169784, Dec 8, 1992

Sep 17, 1990

7/583392

Max D. Summers - Bryan TX
Christian E. G. Oker-Blom - Turku, FI

The Texas A & M University System - College Station TX

C12N 1586

4353201

This invention relates to specifically designing and genetically engineering recombinant baculovirus for producing, in a compatible insect system, a desired protein, virus, protein hybrid, or virus hybrid. In particular aspects, this invention relates to the use of different baculovirus promoters for the ultimate purpose of constructing a recombinant baculovirus designed for the investigator's specific need. For example, the recombinant baculovirus of this invention can be designed to produce a viral pesticide. This invention also describes the construction of a genetically engineered virus or virus hybrid (e. g. animal or human pathogen) which is not capable of replicating itself but is essentially identical to the authentic pathogen in terms of structure and antigenicity. This baculovirus is constructed such that the non-structural viral genes are truncated, mutated or both and are located 3' and directly under the control of an early baculovirus gene promoter and the structural viral genes are located 3' and directly under the control of a late baculovirus gene promoter. This genetically engineered baculovirus is therefore capable of temporal regulation and successive synthesis of non-structural and structural proteins.

4879236, Nov 7, 1989

Apr 16, 1987

7/040367

Gale E. Smith - Bryan TX
Max D. Summers - Bryan TX

The Texas A&M University System - College Station TX

C12N 700
C12N 2100
C12N 1500

435235

A method for producng a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell, is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant transfer vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant transfer vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. The resultant recombinant baculovirus is then used to infect susceptible insects or cultured insect cells and the protein product from the incorporated selected gene is produced from the infection.

4745051, May 17, 1988

May 27, 1983

6/498858

Gale E. Smith - Bryan TX
Max D. Summers - Bryan TX

The Texas A&M University System - College Station TX

C12P 2100
C12P 1934
C12N 1500
C12N 100
C12N 700
C07H 2104

435 68

A method for producing a recombinant baculovirus expression vector, capable of expression of a selected gene in a host insect cell, is disclosed. The preferred method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant shuttle vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant shuttle vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome.

5278050, Jan 11, 1994

Jun 3, 1992

7/892757

Max D. Summers - Bryan TX

The Texas A&M University System - College Station TX

C12P 2102
C12P 2100

435 691

The engineering of foreign vertebrate gene constructs by recombinant DNA techniques for the more efficient processing and secretion of foreign genes in insect systems is done by replacing the foreign vertebrate protein signal peptide sequences with protein signal sequences from insect cell secreted proteins. A baculovirus expression vector system is constructed wherein the natural signal DNA peptide sequence associated with the desired foreign gene, such as CD4 (T cell surface protein T4) is replaced by the signal DNA sequence from the insect signal peptides coding for the cuticle gene or adipokinetic hormone.

5547871, Aug 20, 1996

Jan 25, 1993

8/009265

Bruce C. Black - Yardley PA
Max D. Summers - Bryan TX

American Cyanamid Company - Wayne NJ

C12N 701
C12N 1532
C12N 1585

4352401

Seven heterologous signal sequence are described for use with genes for insect controlling proteins, such that when the signal sequence and protein genes are inserted into an insect virus, that virus demonstrates an earlier onset of morbidity than a wild-type insect virus which lacks the gene for the insect controlling protein.

5155037, Oct 13, 1992

Aug 4, 1989

7/389376

Max D. Summers - Bryan TX

The Texas A&M University System - College Station TX

C12N 500
C12N 1585
C12N 1587
C07H 1512

4352402

The engineering of foreign vertebrate gene constructs by recombinant DNA techniques for the more efficient processing and secretion of foreign genes in insect systems is done by replacing the foreign vertebrate protein signal peptide sequences with protein signal sequences from insect cell secreted proteins. A baculovirus expression vector system is constructed wherein the natural signal DNA peptide sequence associated with the desired foreign gene, such as CD4 (T cell surface protein T4) is replaced by the signal DNA sequence from the insect signal peptides coding for the cuticle gene or adipokinetic hormone.