Inventors:
Om P. Surujballi - Nepean, CA
Anna Romanowska - Ottawa, CA
Michael E. Jolley - Round Lake IL, US
Mohammad Sarwar Nasir - Grayslake IL, US
Assignee:
Diachemix LLC - Milwaukee WI
Her Majesty the Queen in Right of Canada, as represented by the Canadian Food Inspection Agency - Ottawa (Nepean), Ontario
International Classification:
C12Q 1/00
A61K 39/04
G01N 33/566
A61K 38/00
US Classification:
435 4, 435 71, 4352531, 436501, 436543, 436546, 530300, 530350, 424 91, 424 92, 4241301, 4241501, 4241641, 4241841, 4242481
Abstract:
The present invention provides an assay for detection of -infected animals. A tracer, comprising a peptide of protein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific for The tracer comprises a peptide of protein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for to produce a detectable change in fluorescence polarization.