Pratap S Singh, Age 82Richmond, TX

6706861, Mar 16, 2004

Mar 22, 2001

09/815195

Pratap Singh - Wilmington DE
Jianfang Wang - Wilmington DE
Liliana Maria Tejidor - Raleigh NC

Dade Behring, Inc. - Deerfield IL

A61K 3514

530381, 424450, 435 13, 436 69, 436829, 530350, 530380

The present application relates to a method of making liposomes having membrane proteins incorporated therein, the method comprising: providing the membrane protein in solution; providing a solution of preformed liposomes; and incubating the mixture. Prior to the step of providing a solution of preformed liposomes, the liposomes are formed by combining a mixture of phospholipids with a solution of at least one type of unsaturated fatty acid. The methods of the present invention further relate to the method of making a reagent comprising tissue factor reconstituted into preformed liposomes. The method of the present invention for making a tissue factor reagent comprises: providing tissue factor in solution; providing a solution of preformed liposomes comprising a mixture of phospholipids and at least one type of unsaturated fatty acid; and incubating the mixture.

7220842, May 22, 2007

Apr 5, 2004

10/818115

Yi-Feng Zheng - Wilmington DE, US
Pratap Singh - Wilmington DE, US
Dorota Bolle - Newark DE, US

Dade Behring Inc. - Deerfield IL

C07K 17/02
C07D 491/02
G01N 33/535

530405, 546 46, 435 79, 435188, 5303889, 5303898, 530406

Methods, compositions and kits are disclosed directed at haptens, immunogens and immunoassays for buprenorphine (BUP) and nor buprenorphine (norBUP). The method comprises providing in combination in a medium (i) a sample suspected of containing buprenorphine (BUP) or norbuprenorphine (norBUP) and (ii) an antibody raised against an immunogen of buprenorphine (BUP) or norbuprenorphine (norBUP). The medium is examined for the presence of a complex comprising a labeled hapten of buprenorphine (BUP) or norbuprenorphine (norBUP) where the presence of such as complex indicates the presence of the compound in the sample.

7863427, Jan 4, 2011

Apr 12, 2007

11/734661

Yi-Feng Zheng - Wilmington DE, US
Pratap Singh - Wilmington DE, US
Dorota Bolle - Newark DE, US

Siemens Healthcare Diagnostics Inc. - Deerfield IL

C07K 17/02
C07D 491/02

530405, 546 46

Methods, compositions and kits are disclosed directed at haptens, immunogens and immnoassays for buprenorphine (BUP) and nor buprenorphine (norBUP). The method comprises providing in combination in a medium (i) a sample suspected of containing buprenorphine (BUP) or norbuprenorphine (norBUP) and (ii) an antibody raised against an immunogen of buprenorphine (BUP) or norbuprenorphine (norBUP). The medium is examined for the presence of a complex comprising a labeled hapten of buprenorphine (BUP) or norbuprenorphine (norBUP) where the presence of such as complex indicates the presence of the compound in the sample.

8394647, Mar 12, 2013

Feb 17, 2011

13/029786

Bhalchandra Lele - Newark DE, US
Pratap Singh - Wilmington DE, US
Asoke Chatterjee - Chadds Ford PA, US

Siemens Healthcare Diagnostics Inc. - Tarrytown NY

G01N 33/544

436529, 436508, 436509, 436534, 502404

Methods and reagents are disclosed for reducing an amount of non-covalently bound polysaccharide on a support. The method comprises contacting a support comprising both covalently bound polysaccharide and non-covalently bound polysaccharide with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support.

8546148, Oct 1, 2013

Apr 9, 2012

13/442503

Bhalchandra Lele - Newark DE, US
Pratap Singh - Wilmington DE, US
Raphael Bartz - Newark DE, US
Jason A. Kellogg - Bear DE, US

Siemens Healthcare Diagnostics Inc - Tarrytown NY

G01N 21/76
G01N 33/53
C08F 226/06
C08F 226/10
C08F 220/58

436501, 436518, 436534, 526304, 526263, 528363, 528361

A composition for use as an assay reagent comprises a solid support comprising a member of a signal producing system and a coating of a synthetic copolymer. The synthetic copolymer comprises a first polymerized monomer comprising a pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and a second polymerized monomer comprising a pendant moiety comprising at least 1 carbon atoms and at least 2 heteroatoms. In some embodiments the copolymer comprises a polyethylenic backbone comprising the pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and the pendant moiety comprising at least 1 carbon atom and at least 2 heteroatoms.

2005011, Jun 2, 2005

Dec 1, 2003

10/725166

Carsten Schelp - Hockessin DE, US
Pratap Singh - Wilmington DE, US
Michael Trier - Wetter, DE
Harald Ackermann - Marburg, DE
Susan Swann - Newark DE, US
Lee Hall - Hockessin DE, US

G01N033/543

436518000

Methods for quantitatively or qualitatively detecting an analyte in a sample and suitable reagents for this purpose, in particular to a carrier molecule to which one or more analyte-specific binding partner(s) is/are bonded and which possesses additional binding sites for a specific binding partner X or Y which is associated with a component of a signal-generating system, and to the use of such a conjugate, in particular in homogeneous binding tests.

2008010, May 8, 2008

Nov 3, 2006

11/592558

Tie Wei - Bear DE, US
Pratap Singh - Wilmington DE, US
James Duffy - Landenberg PA, US
Amy Posey - Cochranville PA, US

G01N 33/566
G01N 33/531
G01N 33/532
G01N 33/533
G01N 33/534
G01N 33/535
G01N 33/563

436501, 436547, 5303871

Methods and compositions are disclosed for reducing non-specific binding in a binding assay for the determination of an analyte in a sample wherein one of the reagents for conducting the binding assay is an antibody reagent. The methods comprise treating the antibody reagent at a pH of about 2.0 to about 3.5. The method may further comprise treating the antibody reagent with a reducing agent in an amount sufficient to reduce non-specific binding of the antibody reagent. In some embodiments the reducing agent may be a thiol-containing reducing agent or a combination of two or more thiol-containing reducing agents.

2009025, Oct 15, 2009

Apr 15, 2008

12/103245

Sandra A. Lewisch - Bear DE, US
Pratap Singh - Wilmington DE, US
Viral Desai - Newark DE, US
Karen L. Krakowski - Chesapeake City MD, US
James E. Duffy - Landenberg PA, US

SIEMENS HEALTHCARE DIAGNOSTICS INC., A CORPORATION OF CALIFORNIA - Deerfield IL

G01N 33/566
G01N 33/536

436501, 436536

Methods are disclosed for designing an antibody reagent for use in an assay for the detection of an analyte to obtain an optimum assay sensitivity and/or dynamic range. The antibody reagent is a conjugate of a small molecule attached by a spacer group to an antibody for the analyte. The method comprises controlling, in the preparation of the conjugate, reaction parameters comprising the hydrophobicity or hydrophilicity of the spacer group, the length of the spacer group, the number of molecules of the small molecule attached to the antibody and the point of attachment of the small molecule to the antibody to obtain an optimum assay sensitivity and/or dynamic range. In some embodiments the method comprises preparing two or more conjugates by selecting a set of parameters for each conjugate wherein the set of parameters is different for each conjugate, conducting an assay for the analyte employing each conjugate and selecting for use in the assay the conjugate that provides the optimum assay sensitivity and/or dynamic range.