Robert Joseph Levis, Age 634 Locust Ln, Rose Valley, PA 19086

8598521, Dec 3, 2013

Aug 17, 2010

13/390722

Robert J. Levis - Rose Valley PA, US
John J. Brady - Philadelphia PA, US
Elizabeth J. Judge - Seaville NJ, US

Temple University of the Commonwealth System of Higher Education - Philadelphia PA

H01J 49/26

250288, 250281, 250282

Methods and apparatus for analyzing samples are disclosed. The samples are analyzed by vaporizing molecules from a sample in a sample area with a femtosecond laser beam under ambient conditions, ionizing the vaporized molecules with electrospray ionization under the ambient conditions to form ions; and analyzing and detecting the ions.

2004012, Jul 1, 2004

Dec 18, 2002

10/322693

Herschel Rabitz - Lawrenceville NJ, US
Elmar Schreiber - Bremen, DE
Robert Levis - Rose Valley PA, US

G06F019/00
G01N031/00

702/023000, 702/027000

The disclosed invention is related to the field of quantum dynamic discriminators, sample identification systems, mass spectrometers and methods for identifying a component in a composition. Also disclosed are quantum dynamic discriminators and methods for ascertaining the quantum dynamic states of a component in a composition. Optimal identification devices and methods for ascertaining quantum Hamiltonians of quantum systems are further disclosed.

2013032, Dec 5, 2013

Feb 3, 2012

13/983451

Robert J. Levis - Rose Valley PA, US
Johanan H. Odhner - Huntingdon Valley PA, US

TEMPLE UNIVERSITY - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION - Philadelphia PA

G01J 3/44

356301

In apparatus and methods of Raman spectroscopy in air, a target region is excited by a laser pump pulse exceeding the critical power for self-focusing in air and having a duration after self-focusing of 15 fs or less. A laser probe pulse having a duration in the range of 200 fs to 100 ps and an energy of at least 20 μJ is directed at the excited target region. Stimulated Raman scattering from the interaction between the excited target region and the laser probe pulse is detected. The target region can be outside the spectrometer, with ambient air in between used for the self-focusing.

5580733, Dec 3, 1996

Sep 6, 1994

8/301732

Robert J. Levis - Grosse Pointe Park MI
Louis J. Romano - Detroit MI

Wayne State University - Detroit MI

C12Q 168
C12P 1934
G01N 3348
B01D 5944

435 6

Method for analyzing a nucleic acid molecule, without fragmenting the molecule, by vaporizing a mixture of the molecule and a matrix by illuminating the mixture with visible laser light absorbed by the matrix and not by the nucleic acid molecule. The method is useful for determining the nucleotide sequence of a polynucleotide by using mass spectrometry to determine the molecular weights of individual single-stranded nucleic acid molecules in a population including a plurality of single-stranded nucleic acid molecules generated from the polynucleotide, each molecule having a different molecular weight, and one defined terminus and one variable terminus which terminates at a specific nucleotide.

5210412, May 11, 1993

Jan 31, 1991

7/648282

Robert J. Levis - Grosse Pointe Park MI
Louis J. Romano - Detroit MI

Wayne State University - Detroit MI

B01D 5944
H01J 4940

250288

Described is a method and apparatus for analyzing an organic sample. In the preferred embodiment, this method and apparatus allows the determination of the base sequence of a nucleic acid by determining the molecular weights of the components of a biological sample. The method uses either a pre-existing chromophore or the covalent attachment of an ionizable chromophore to a biological sample followed by the vaporization of these molecules by exposure to an intense pulse of electromagnetic radiation in the presence of a matrix which strongly absorbs the radiation. The gaseous molecules are subsequently extracted into an evacuated ionization chamber and then exposed to electromagnetic radiation at a wavelength which specifically excites the chromophore covalently attached to the biological sample. The molecular weights of these ionized species are then determined by mass spectroscopic analysis. This method of molecular weight determination allows for a DNA sequencing method.

2015018, Jul 2, 2015

Dec 27, 2013

14/142240

- Ann Arbor MI, US
Martin E. FERMANN - Dexter MI, US
Jiahui PENG - Ann Arbor MI, US
Robert J. LEVIS - Rose Valley PA, US

IMRA AMERICA, INC. - Ann Arbor MI

H01J 49/16
G01N 30/96
G01N 33/483
G01N 33/50
G01N 33/68
G01N 33/92
G01N 33/94
G01N 33/02
H01J 49/04
G01N 33/49

The present invention relates to a mass spectrometer system, which combines laser desorption with pulse bursts comprising a train of ultrashort pulses and electrospray ionization. The pulse separation between individual pulses within the pulse burst is selected such that transient phenomena on an irradiated sample do not fully relax between individual pulses. Pulses with pulse widths ranging from fs to sub ns are conveniently implemented. The pulse widths can be selected to allow for multi-photon excitation of a sample while at the same time minimizing heat accumulation in a sample. Low cost laser systems such as fiber lasers can be configured to generate appropriate pulse bursts. The technique is suitable for mass spectrometry imaging with high spatial resolution. The laser system can serve as an electronic clock to which the whole mass spectrometry system or mass spectrometry imaging system is synchronized.