Michael A Rose, Age 84Walnut Creek, CA

6369199, Apr 9, 2002

Mar 5, 2001

09/799645

Karl Guegler - Menlo Park CA
Ruoying Tan - Foster City CA
Michael J. Rose - Palo Alto CA

Incyte Genomics, Inc. - Palo Alto CA

C07K 1900

530350

Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

6436676, Aug 20, 2002

Nov 1, 2001

10/002528

Karl Guegler - Menlo Park CA
Ruoying Tan - Foster City CA
Michael J. Rose - Palo Alto CA

Incyte Genomics, Inc. - Palo Alto CA

C12P 1934

435 911, 435 913, 435 915

Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

6703239, Mar 9, 2004

Jun 25, 2002

10/180196

Karl Guegler - Menlo Park CA
Ruoying Tan - Foster City CA
Michael J. Rose - Palo Alto CA

Incyte Corporation - Palo Alto CA

C12N 510

435325, 435243, 4353201, 435419, 536 234

Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

2002012, Sep 12, 2002

Mar 9, 2001

09/803317

Ruoying Tan - Foster City CA, US
Glenn Fu - Dublin CA, US
Michael Rose - Mountain View CA, US
Juan Zhang - Cupertino CA, US

C12Q001/68
G01N033/53
C12Q001/02
C12N015/00

435/006000, 435/007100, 435/320100, 435/029000

The present invention provides a method in which cDNAs that encode signal sequences for secreted or membrane-associated proteins are isolated using a fusion protein that directs secretion of a molecule that provides antibiotic resistance, e.g., -lactamase. The present method allows the isolation of signal peptide-associated proteins that may be difficult to isolate with other techniques. Moreover, the present method is amenable to throughput screening techniques and automation, and especially in validating the presence of the signal sequence via expression of the protein in both prokaryotic and eukaryotic cells. This invention provides a powerful and approach to the large scale isolation of novel secreted proteins.

2011006, Mar 17, 2011

Sep 16, 2009

12/586131

Shelley Force Aldred - Hayward CA, US
Michael Rose - Walnut Creek CA, US
Patrick Collins - Millbrae CA, US
Nathan D. Trinklein - Redwood City CA, US

SwitchGear Genomics, Inc., a Delaware Corporation - Menlo Park CA

C12Q 1/68
C40B 40/06
C40B 40/02
C12N 5/00
C40B 60/00

435 6, 506 16, 506 14, 435325, 506 33

This invention provides transcription regulatory control sequences, the activity of which function as biomarkers for a variety of biological responses. This invention also provides expression constructs in which a biomarker transcription regulatory sequence is operably linked with a sequence for a reporter. Cells that comprise these expression constructs can be used in assays to identify conditions that modulate activity of the biological response.

6326175, Dec 4, 2001

Jul 13, 1999

9/352540

Karl Guegler - Menlo Park CA
Ruoying Tan - Foster City CA
Michael J. Rose - Palo Alto CA

Incyte Pharmaceuticals, Inc. - Palo Alto CA

C12P 1934

435 9151

Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length CDNA libraries are employed.

6083727, Jul 4, 2000

Jun 30, 1999

9/343945

Karl Guegler - Menlo Park CA
Ruoying Tan - Foster City CA
Michael J. Rose - Palo Alto CA

Incyte Pharmaceuticals Inc. - Palo Alto CA

C07H 2102
C07H 2104
C12P 1934
C12Q 168

435 915

Methods are provided for preparing a double-stranded cDNA corresponding to the 5' end of an mRNA. In the subject methods, an mRNA is first contacted with an oligo dT primer under first strand cDNA synthesis conditions. Next, the resultant hybrid is contacted with a random primer under first strand cDNA synthesis conditions, such that a cDNA complementary to the 5' end of the mRNA is produced. The resultant hybrid molecule is converted to two different double-stranded cDNAs, a first cDNA comprising the oligo dT primer and a second cDNA lacking the oligo dT primer. The two double-stranded cDNAs are then separated from each other. The subject methods find use in a variety of applications, and find particular use in the synthesis of 5' enriched cDNA libraries. Also provided are cDNA libraries produced by the subject methods, as well as kits for performing the subject methods.