Saiful I ChowdhuryDallas, TX

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Dallas, TX

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President

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Saiful Chowdhury

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Us Patents

Dual Mass Spectometry-Cleavable Crosslinking Reagents For Protein-Protein Interactions

US Patent:
2020035, Nov 12, 2020
Filed:
Jul 8, 2020
Appl. No.:
16/923811
Inventors:
- Austin TX, US
Saiful Mahmud Chowdhury - Bedford TX, US
International Classification:
C07K 5/103
C07K 5/093
C07K 7/06
G01N 33/68
Abstract:
Described are dual mass-spectrometry-cleavable cross-linkers that can be cleaved selectively using two differential tandem mass-spectrometric techniques such as collision induced dissociation (CID) or electron transfer dissociation (ETD), i.e., a dual cleavable crosslinking technology (DUCCT) cross-linker. When used to cross-link a macromolecule, such as a peptide, MS/MS fragmentation produces two signature complementary mass spectra of same cross-linked peptides, the analysis of which gives rise to high confidence in characterizing the structures of the cross-linked macromolecules as well as sites of interactions. Also described, are methods of making and using DUCCT cross-linkers.

Dual Mass Spectrometry-Cleavable Crosslinking Reagents For Protein-Protein Interactions

US Patent:
2020000, Jan 2, 2020
Filed:
Jun 5, 2017
Appl. No.:
16/307375
Inventors:
- Austin TX, US
Saiful Mahmud Chowdhury - Bedford TX, US
International Classification:
C07K 5/103
G01N 33/68
C07K 7/06
C07K 5/093
Abstract:
Described are dual mass-spectrometry-cleavable cross-linkers that can be cleaved selectively using two differential tandem mass-spectrometric techniques such as collision induced dissociation (CID) or electron transfer dissociation (ETD), i.e., a dual cleavable crosslinking technology (DUCCT) cross-linker. When used to cross-link a macromolecule, such as a peptide, MS/MS fragmentation produces two signature complementary mass spectra of same cross-linked peptides, the analysis of which gives rise to high confidence in characterizing the structures of the cross-linked macromolecules as well as sites of interactions. Also described, are methods of making and using DUCCT cross-linkers.

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