Shiv Kumar, Age 6078 Spenser Dr, Short Hills, NJ 07078

6444682, Sep 3, 2002

Apr 18, 2000

09/463501

Adrian Christopher Simmonds - Amersham, GB
Alan Hamilton - Amersham, GB
Clifford Smith - Tring, GB
David Loakes - Letchworth, GB
Daniel Brown - Cambridge, GB
Fergal Hill - Cambridge, GB
Shiv Kumar - Belle Mead NJ
Satyam Nampalli - Belle Mead NJ
Mark McDougall - Bethlehem PA

Nycomed Amersham PLC - Buckinghamshire

A61K 3170

514274, 514269, 514470, 514471, 514414, 514 23, 536 221, 544299, 544301, 544302, 544304

Nucleotide or base analogues having structure (3) or (4) wherein X O or NH or S and each R is independently H or alkyl or alkenyl or alkoxy or aryl or a reporter moiety.

6605611, Aug 12, 2003

Sep 3, 2002

10/233993

Adrian Christopher Simmonds - Amersham, GB
Alan Hamilton - Amersham, GB
Clifford Smith - Tring, GB
David Loakes - Letchworth, GB
Daniel Brown - Cambridge, GB
Fergal Hill - Cambridge, GB
Shiv Kumar - Belle Mead NJ
Satyam Nampalli - Belle Mead NJ
Mark McDougall - Bethlehem PA

Nycomed Amersham PLC - Little Chalfont

A61K 31495

514274, 514248, 5142621, 5142641, 514269, 514461, 514 23, 544236, 544256, 544279, 544298, 544315, 546118, 546121, 546123, 536 221

Nucleoside analogues or base analogues having structure (I), where X=O or NH or S, Y=N or CHR or CR , W=N or NR or CHR or CR or S, n=1 or 2; each R is independently H or O or alkyl or alkenyl or alkoxy or aryl or a reporter moiety; where necessary (i. e. when Y and/or W is N or CR where R is not O) a double bond is present between Y and W or W and W, and Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue.

6664058, Dec 16, 2003

Jul 3, 2001

09/898210

Shiv Kumar - Belle Mead NJ
Satyam Nampalli - Belle Mead NJ
Constantin Neagu - West Windsor NJ
Mark McDougall - Arroyo Grande CA
David Loakes - Cambridge, GB
Dan Brown - Cambridge, GB

Amersham Biosciences UK Limited - Buckinghanshire
Medical Research Council Laboratories of Molecular Biology - Cambridge

C12Q 168

435 6, 435 911, 435 912, 536 231, 536 253

The present invention describes novel compounds of the formula Wherein Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue, Y=O, S, NR , where R is H, alkyl alkenyl, alkynyl, X is H, alkyl, alkenyl, alkynyl, aryl, heteroaryl or a combination thereof or, preferably, a reporter group. The novel compounds are suitable for incorporation in oligonucleotides and polynucleotides.

6855503, Feb 15, 2005

Dec 20, 2002

10/326039

Satyam Nampalli - Belle Mead NJ, US
Weihong Zhang - Highland Park NJ, US
Sudhakar Rao - Belle Mead NJ, US
Shiv Kumar - Belle Mead NJ, US

Amersham Biosciences Corp - Piscataway NJ

C12Q001/68
C07D001/02

435 6, 536 231, 544 1

Exploitation of suitably functionalized heterocyclic molecules, in the design and synthesis of Fluorescence Resonance Energy Transfer (FRET) cassettes and their corresponding dideoxynucleotide terminators culminated into efficient reagents for DNA sequencing. Additionally, these FRET cassettes/terminators, of the present invention, derived from different classes of heterocyclic systems have high potential to be used for general labelling of biological molecules to generate highly sensitized signals. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.

6967250, Nov 22, 2005

Aug 30, 1999

09/386576

Shiv Kumar - Belle Mead NJ, US
Satyam Nampalli - Belle Mead NJ, US
Mahesh Khot - Edison NJ, US

Amersham Biosciences Corp - Piscataway NJ

C07D265/36

544105, 435 6, 549223, 549224, 549227, 536 266

Energy transfer dyes, their preparation, and their use as labels in biological systems is disclosed. The dyes are preferably in the form of cassettes which enable their attachment to a variety of biological materials. The dyes and the reagents that can be made from them offer a wide variety of fluorescent labels with large Stokes' shifts enabling their use in a variety of fluorescence applications over a wide range of the visible spectrum.

7033762, Apr 25, 2006

Apr 1, 2002

10/113025

John Nelson - Neshanic Station NJ, US
Carl Fuller - Berkeley Heights NJ, US
Anup Sood - Flemington NJ, US
Shiv Kumar - Belle Mead NJ, US

Amersham Biosciences Corp - Piscataway NJ

C12Q 1/68

435 6

A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, a non-hydrolyzable primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and an enzyme having exonuclease activity which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species characteristic of the sample; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on the detection.

7041812, May 9, 2006

Aug 29, 2002

10/230576

Shiv Kumar - Belle Mead NJ, US
Anup Sood - Flemington NJ, US

Amersham Biosciences Corp - Piscataway NJ

C07H 21/00
C07H 21/02
C07H 21/04
C12Q 1/68
C12P 19/34

536 231, 435 6, 435 911, 435 912, 536 243

The present invention describes new compositions of matter in the form of labeled nucleoside polyphosphates with four or more phosphates. In addition compositions of nucleoside polyphosphates with four or more phosphates that are substrates for nucleic acid polymerases with enhanced substrate properties and methods of using these nucleoside polyphosphates for nucleic acid detection, characterization and quantification are described. The compositions provided by this invention include nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogues which have colorimetric, chemiluminescent, or fluorescent moieties, mass tags or an electrochemical tags attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Removal of the polyphosphate product of phosphoryl transfer via phosphate or polyphosphate transferring enzyme leads to a detectable change in the label attached thereon.

7052839, May 30, 2006

Apr 1, 2002

10/113030

John Nelson - Neshanic Station NJ, US
Carl Fuller - Berkeley Heights NJ, US
Anup Sood - Flemington NJ, US
Shiv Kumar - Belle Mead NJ, US

Amersham Biosciences Corp - Piscataway NJ

C12Q 1/68

435 6

The present invention describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.