Xuan LiCity Of Industry, CA

2021029, Sep 23, 2021

Jun 7, 2021

17/340581

- Oakland CA, US
Xuan Li - Irvine CA, US
Neha Garg - Irvine CA, US
Yu-Hsi Chen - Irvine CA, US

B01L 3/00
C12N 15/87

The present invention features the use of cavity acoustic transducers (CATs) to apply mechanical stimuli on cells. CATs utilize the generated acoustic microstreaming vortices to trap cells and apply tunable shear on them. The present invention may use such a portable, automated, and high throughput device for cell transfection.

2021003, Feb 11, 2021

Aug 7, 2020

16/988179

- Oakland CA, US
Xuan Li - Irvine CA, US

B01L 3/00

A microfluidic device having an array for cell trapping is used to analyze cell-cell interaction at single-cell level. The microfluidic trapping array efficiently pairs single cells in isolated compartments in an easy-to-operate manner. A first cell is squeezed through an opening of a first cavity by a strong forward flow. Subsequently, a second cell is pushed into a second cavity by a low reverse flow. The trapped cell pairs are sealed by an oil phase or hydrogel into isolated compartments, thereby eliminating interference from other cell pairs or the surrounding media.

2020023, Jul 30, 2020

Apr 13, 2020

16/847305

- Oakland CA, US
- Johnston IA, US
Dohyun Lee - Irvine CA, US
Xuan Li - Irvine CA, US
Ning Ma - Irvine CA, US
Yue Yun - Johnston IA, US

B01L 3/00
C12Q 1/02
C12N 15/10

Methods and devices for single cell analysis using fluorescence lifetime imaging microscopy (FLIM) are disclosed. The methods utilize microfluidic devices which use traps to immobilize cells for FLIM analysis. The analysed cells may be sorted before or after imaging and may be plant, animal, or bacterial cells. Analysis of the FLIM data may use a phasor plot and may be used to identify a metabolic pattern of the single cells.

2020010, Apr 9, 2020

Dec 9, 2019

16/707560

- Oakland CA, US
Gopakumar Kamalakshakurup - Irvine CA, US
Xuan Li - Irvine CA, US
Gisela Lin - Irvine CA, US
Xuhao Luo - Irvine CA, US
Marzieh Ataei - Irvine CA, US
Michelle A. Digman - Irvine CA, US
Francesco Palomba - Irvine CA, US

B01L 3/00
C12N 11/00
G01N 21/64

A passive, hydrodynamic technique implemented using a microfluidic device to perform co-encapsulation of samples in droplets and sorting of said droplets is described herein. The hydrodynamic technique utilizes laminar flows and high shear liquid-liquid interfaces at a microfluidic junction to encapsulate samples in the droplets. A sorting mechanism is implemented to separate sample droplets from empty droplets. This technique can achieve a one-one-one encapsulation efficiency of about 80% and can significantly improve the droplet sequencing and related applications in single cell genomics and proteomics.

2020006, Feb 27, 2020

Aug 21, 2019

16/547152

- Oakland CA, US
Xuan Li - Irvine CA, US
Neha Garg - Irvine CA, US

B01L 3/00
C12N 15/87

The present invention features the use of lateral cavity acoustic transducers (LCATs) to apply mechanical stimuli on cells. LCATs utilize the generated acoustic microstreaming vortices to trap cells and apply tunable shear-induced cell deformation on them. The present invention may use such a portable, automated, and high throughput device for shear-induced cell transfection.

2018023, Aug 16, 2018

Apr 11, 2018

15/950982

- Oakland CA, US
- Johnston IA, US
H. Kumar Wickramasinghe - Irvine CA, US
Yinglei Tao - Irvine CA, US
Xuan Li - Irvine CA, US
Yue Yun - Johnston IA, US

C12N 15/10
C12Q 1/6806
B01L 3/00
C12Q 1/6844

Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.

2018007, Mar 22, 2018

Sep 7, 2017

15/697898

- Oakland CA, US
H. Kumar Wickramasinghe - Irvine CA, US
Do-Hyun Lee - Irvine CA, US
Yinglei Tao - Irvine CA, US
Xuan Li - Irvine CA, US

B01L 3/00
C12Q 1/68
C12N 15/10

Disclosed is a lab-on-a-chip platform comprising a microfluidic device sealed with a thin membrane. An analysis probe configured to penetrate the thin membrane to access a sample trapped in the microfluidic device.

2017010, Apr 20, 2017

Oct 7, 2016

15/288969

- Oakland CA, US
- Johnston IA, US
H. Kumar Wickramasinghe - Irvine CA, US
Yinglei Tao - Irvine CA, US
Xuan Li - Irvine CA, US
Yue Yun - Johnston IA, US

C12N 15/10
C12Q 1/68

Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.