Work:
Brigham and Women's Hospital/Cardiovascular Division Jun 2011 to 2000
Postdoctoral Research Fellow
Boston University School of Medicine/Oncology Department - Boston, MA Sep 2006 to May 2012
Graduate Student
Tongji Medical College/Surgery Department - Wuhan, CN Sep 2002 to Sep 2006
Graduate Student
Tongji Hospital of Tongji Medical College - Wuhan, CN Sep 2000 to Sep 2002
Medical Student
Education:
Boston University 2006 to 2012
Doctor of Philosophy in Molecular Medicine Program
Tongji Medical College 2002 to 2007
Doctor of Medicine in Oncology
Tongji Medical College 1997 to 2002
Bachelor of Medicine
Skills:
Molecular Biology: DNA/RNA isolation, purification and amplification. PCR cloning with TA/Gateway technology. Co-immunoprecipitation and GST pull down assay. Quantitative PCR with SYBR Green technique. Construct design and cloning to achieve overexpression and knockdown (shRNA) of targeted genes in cell lines and animals via adenovirus and lentivirus. Western blotting, In situ Western blotting, ELISA, Flow cytometry, in vitro transcription, protein engineering, exosome isolation and characterization Cellular Biology: Culture of cancer cell lines (HepG2, Hep3B, MCF7, HEK cells). Establishment of stable transfected cell line. In vitro cancer assay: proliferation assay (Staining with proliferation markers: Ki67, Phospho-Histone 3; Flow cytometry with DAPI staining for cell cycle analysis; High-through put screening with CellTiter Glo luminescent assay); cell death assay (Caspase 3 immunoblotting, Flow cytometry assay with Annexin 5 staining, MTT assay); cell function assay: Mitochondrial function assay and lysotracker function. Cellular and Animal Imaging: Harvesting, fixing, trimming, processing, embedding, sectioning of animal and human samples. Immunohistochemistry staining. Immunofluorescence staining, Laser scanning confocal microscopy (running the confocal imaging core in CMRL), living cell imaging, organelle imaging; 2D echocardiography and color Doppler in cardiac imaging and function assessment. Whole animal imaging and non-invasive tracking of implanted cells. In Vivo: Collection of serum/plasma/whole blood, tissues, primary tumor material and metastases from animal models. Mouse anesthesia and surgery. Tumor xenotransplantation in immunosuppressed nude mice, Pharmacological agent administration to mice or rats via IV, IP, SC, IM and PO. Establishment of plasma cell tumor transgenic mouse model. Software: GraphPad Prism, SPSS.
