Jianming Xiao, Age 61348 Dana St, Fremont, CA 94539

2002016, Nov 7, 2002

Feb 19, 2002

10/080274

Jianming Xiao - Fremont CA, US
Shiping Chen - Fremont CA, US
Yuling Luo - Castro Valley CA, US
John Butler - San Jose CA, US
Masoud Qazan - Castro Valley CA, US
Jochen Thiele - Mountain View CA, US
Anthony Chen - Sunnyvale CA, US

G01N033/545
C12M003/00

436/524000, 422/102000, 422/100000, 436/531000, 435/287200, 435/288400, 216/002000

Disclosed are systems and methods involved in extreme high throughput screening of compounds which have an affinity for a biological target. The system is based on a capillary bundle with two distinguishable ends wherein capillaries on one end are connected to compounds stored in discrete reservoirs and capillaries on the other end are bound and processed to form a two dimensional microarray. A capillary bundle having reaction wells for hybridization and compound reaction in one end of the capillaries is disclosed. The capillaries may be light-conducting. Also disclosed are various methods of identifying a target compound in a liquid using this capillary bundle as well as methods of fabricating the bundle. A novel surface tension guided reaction chamber is also provided. Methods and chemistry for fabrication and use of a surface tension guided reaction chamber in binding and hybridization assays are also disclosed. Methods and systems for precise metering of fluids within the capillaries and at the reaction chambers, including the surface tension guided “virtual” reaction well is provided. Methods for performing high throughput screens using optical fiber lined capillaries of the invention are also provided. The capillary arrays are used with both liquid probes as well as probes immobilized on the walls of the capillary.

2003008, May 8, 2003

Oct 4, 2002

10/264892

Shiping Chen - Fremont CA, US
Jianming Xiao - Fremont CA, US
Kaijun Li - San Jose CA, US
Hoang Nguyen - San Jose CA, US

C12Q001/68
C12M001/34

435/006000, 435/287200, 435/286500

Methods and apparatus for promoting interactions between an array of probes deposited on a microarray substrate and target molecules in a target liquid are provided. A microarray apparatus can include a substrate having an array of probes deposited on a surface of the substrate for interaction with a target molecule in a target liquid. The apparatus also includes a cover coupled to the substrate to form a reaction chamber therebetween, wherein the array of probes is contained within the reaction chamber and the substrate and the cover are movable relative to each other.

2003012, Jul 3, 2003

Nov 14, 2002

10/295230

Shiping Chen - Fremont CA, US
Yuling Luo - Castro Valley CA, US
Anthony Chen - Sunnyvale CA, US
Theo Nikiforov - San Jose CA, US
Jianming Xiao - Fremont CA, US
Quan Nguyen - Pleasant Hills CA, US

C12Q001/68
G01N033/53
C12M001/34

435/006000, 435/007100, 435/287200

Systems and methods of biochemical analysis are provided. A method of performing biochemical assays includes loading a first plurality of reservoirs on a first liquid carrier with a first plurality of compounds, coupling a second carrier with the first carrier, the second carrier having a second plurality of reservoirs configured to couple with the first plurality of reservoirs and containing a second plurality of compounds, transferring at least a portion of the first plurality of compounds to the second plurality of reservoirs, and separating at least one component from the second plurality of reservoirs. A system for biochemical analysis includes a first carrier and a second carrier. The first carrier includes a first substrate and a plurality of reservoirs in the substrate for retaining a first plurality of compounds. The second carrier includes a second substrate and a plurality of projections, each projection having a distal end provided with a receiving feature for receiving a component from the plurality of reservoirs when the first carrier and the second carrier are coupled.

2002002, Mar 7, 2002

Feb 22, 2001

09/791410

Jianming Xiao - Fremont CA, US
Shiping Chen - Rockville MD, US
Yuling Luo - Castro Valley CA, US
Anthony Chen - Sunnyvale CA, US

B01J019/00

422/100000, 422/099000, 422/102000, 422/130000, 422/131000

Disclosed are systems and methods involved in ultra high throughput screening of chemical compounds which have an affinity for or interact with a biological target. The invention utilizes an apparatus for ultra high throughput screening of chemical compounds for a biological target. The system is based on a capillary bundle that has two distinguishable ends. Capillaries on one end are connected to chemical compounds stored in discrete reservoirs such as micro titer plate wells; capillaries on the other end are tightly bound and then processed to form a two dimensional array that enable the parallel reactions of a target and many different compounds.