John G Doench, Age 4636 Robinwood Ave APT 3, Boston, MA 02130

2005026, Dec 1, 2005

Dec 5, 2004

11/005127

John Doench - Somerville MA, US
Derek Dykxhoorn - Watertown MA, US
Carl Novina - Newton MA, US
Phillip Sharp - Newton MA, US

C12P021/06
C12N005/06
C12N005/08
C12N015/85

435358000, 435455000, 435367000

The present invention provides reagents such as cells, cell lines, and vectors, that can be used to identify mammalian genes whose expression products (RNA or protein) play a role in RNA interference (RNAi) and/or to identify chemical modulators of RNAi, or for other purposes. The invention further provides a variety of methods for identifying such genes or modulators. In particular, the invention provides a mammalian cell comprising a nucleic acid that encodes a selectable marker and one or more nucleic acid templates for transcription of an RNAi-inducing agent integrated into the genome of the cell, wherein the RNAi-inducing agent reduces expression of the marker and is not naturally found in the cell. Additional cells and cell lines comprising nucleic acids that encode one or more additional markers are also provided. According to certain of the inventive methods cells such as these are mutagenized, transfected or infected with a library of genetic suppressor elements, or contacted with a test compound. Cells in which RNAi is inhibited or activated are identified using an appropriate selective condition or screening method. The identity of the mutated or inhibited gene or the identity of the compound is then determined.

2021010, Apr 15, 2021

Sep 21, 2020

17/027151

- Cambridge MA, US
Neville Espi SANJANA - Cambridge MA, US
John DOENCH - Cambridge MA, US
David ROOT - Cambridge MA, US
Ella Nicole BIEWENER HARTENIAN - Cambridge MA, US

The Broad Institute, Inc. - Cambridge MA
Massachusetts Institute of Technology - Cambridge MA

C12N 15/10
C12N 15/63
C12N 9/22
C12N 15/90

The present invention generally relates to libraries, compositions, methods, applications, kits and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.

2020028, Sep 10, 2020

Mar 4, 2020

16/809458

- Cambridge MA, US
John Doench - Cambridge MA, US
JT Neal - Cambridge MA, US
Jesse Boehm - Cambridge MA, US
Oana Ursu - Cambridge MA, US

C12Q 1/6869
C12N 15/10

The present invention provides methods and tools for analyzing genetic interactions. The subject matter is generally directed to single-cell genomics and proteomics, including methods of performing genome-wide CRISPR perturbation screens and determining gene expression phenotypes.

2016027, Sep 22, 2016

Dec 17, 2015

14/973062

- Cambridge MA, US
Neville Espi Sanjana - Cambridge MA, US
John Doench - Boston MA, US
David Root - Brookline MA, US
Ella Nicole Biewener Hartenian - Brookline MA, US

C12N 15/10
C12N 15/90

The present invention generally relates to libraries, compositions, methods, applications, kits and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.