John B Findlay, Age 76Fernandina, FL

6645758, Nov 11, 2003

Sep 28, 1992

07/951616

Paul Nicholas Schnipelsky - Rochester NY
Leonard Joseph Seaberg - Penfield NY
Charles Cullis Hinckley - Pittsford NY
Jeffrey Allen Wellman - Rochester NY
William Harold Donish - Rochester NY
John Bruce Findlay - Rochester NY

Johnson Johnson Clinical Diagnostics, Inc. - Rochester NY

C12M 118

4352872, 422 58, 422 681, 422100, 422102, 4352876, 4352885

A cuvette and a method of use prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are either pre-incorporated into compartments in the cuvette or added after amplification. In the latter case, a check valve prevents amplified nucleic acid from being released. Transfer of liquids between compartments is achieved via the use of flexible compartment walls and an external pressure source, or via pistons that are part of the cuvette and operate on the compartments as a piston within a piston chamber.

6709813, Mar 23, 2004

May 14, 1993

08/062021

Lynn Bergmeyer - Rochester NY
Thomas J. Cummins - Rochester NY
John Bruce Findlay - Rochester NY
JoAnne H. Kerschner - Rochester NY

Ortho-Clinical Diagnostics, Inc. - Rochester NY

C12Q 168

435 6, 435 912, 514 44, 536 2433

An aqueous composition containing primers for opposing strands of human cytomegaloviral DNA and a second target DNA can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T within the range of from about 65 to about 74Â C. , while the T s are within about 5Â C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of hCMV DNA and other target DNAs using multiple capture probes, in âmultiplexingâ. All of the capture probes have T s which are greater than 50Â C. and are within 15Â C. of each other.

6936415, Aug 30, 2005

Sep 29, 2000

09/675828

Thomas J. Cummins - Rochester NY, US
Susan Melissa Atwood - Rochester NY, US
Lynn Bergmeyer - Rochester NY, US
John Bruce Findlay - Rochester NY, US
John W. H. Sutherland - Rochester NY, US
JoAnne H. Kerschner - Rochester NY, US

C12Q001/68
C12P019/34
C07H021/02
C07H021/04

435 6, 435 912, 536 231, 536 243

An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a Twithin the range of from about 65 to about 74 C. , while the T's are within about 5 C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in “multiplexing”, using multiple capture probes. All of the capture probes have T's which are greater than 50 C. and are within 15 C. of each other.

4547461, Oct 15, 1985

Jan 18, 1983

6/459026

Theodore W. Esders - Webster NY
Shirley Y. Lynn - Rochester NY
John B. Findlay - Rochester NY
Richard M. Schubert - Spencerport NY

Eastman Kodak Company - Rochester NY

C12Q 129
C12Q 150
C12Q 148
C12Q 126
C12Q 128
C12N 912
G01N 148
G01N 2106

435 17

An enzymatic method for the analytical determination of creatine kinase in an aqueous liquid such as blood serum is described. The determination is made by measuring an optical density change using the reagents creatine phosphate, adenosine diphosphate, glycerol, glycerol kinase,. alpha. -glycerophosphate oxidase, a chromagen and a mercapto-containing creatine kinase activator. The mercapto-containing creatine kinase activator is added in encapsulated form or in low concentrations so as to preserve the activity of the chromagen.

5262297, Nov 16, 1993

Apr 30, 1992

7/876672

Richard C. Sutton - Rochester NY
Susan J. Danielson - Rochester NY
John B. Findlay - Rochester NY
Marsha D. B. Oenick - Rochester NY
Ignazio S. Ponticello - Pittsford NY
Harold C. Warren - Rush NY

Eastman Kodak Company - Rochester NY

G01N 83545
G01N 33546
G01N 33547

435 5

Specific binding methods are used for diagnostic assays and purification separations whereby the specific binding capture reagent is prepared from copolymers having highly reactive carboxy groups. These groups are extended from the polymer surface with a linking group having from 8 to 50 atoms in the chain and two or more alkylene, arylene, alkylenearylene or arylenealkylene groups. To these reactive groups is attached a biologically active substance such as a protein or oligonucleotide which then participates in the diagnostic assays or purification separation methods.

5147777, Sep 15, 1992

Jun 18, 1990

7/539774

Richard C. Sutton - Rochester NY
Susan J. Danielson - Rochester NY
John B. Findlay - Rochester NY
Fred T. Oakes - Rochester NY
Marsha D. B. Oenick - Rochester NY
Ignazio S. Ponticello - Pittsford NY
Harold C. Warren - Rush NY

Eastman Kodak Company - Rochester NY

G01N 33545
G01N 33546

435 5

Biologically active reagents are prepared from particles of copolymers having highly reactive carboxy or equivalent groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through highly reactive carboxy groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods as affinity chromatography reagents.

5674717, Oct 7, 1997

Oct 25, 1995

8/548078

John W. Backus - Williamson NY
William Harold Donish - Rochester NY
John Bruce Findlay - Rochester NY
John William H. Sutherland - Rochester NY
Marlene M. King - Penfield NY

Johnson & Johnson Clinical Diagnostics, Inc. - Rochester NY

C12P 1934
C12Q 168

435 912

Nucleic acids can be amplified and detected using a very rapid polymerase chain reaction procedure in which two different nucleic acid sequences are present. This method allows one to preferentially modulate (for example, suppress) the degree of amplification of one or more nucleic acid sequences relative to other nucleic acid sequences. This modulation is achieved by exploiting differences in the relative primer melt temperatures, or by using certain ratios of primers. Each PCR cycle is very fast, that is less than about 90 seconds. This method is particularly useful for amplification and detection of DNA associated with infectious agents that may be present in a specimen in very small quantities compared to other nontargeted nucleic acids.

5888723, Mar 30, 1999

Nov 20, 1992

7/980512

Richard Calvin Sutton - Rochester NY
Ignazio Salvatore Ponticello - Pittsford NY
Thomas Joseph Cummins - Rochester NY
Dennis Roland Zander - Penfield NY
William Harold Donish - Rochester NY
Paul Hong-Dze Chen - Oak Brook IL
John Bruce Findlay - Rochester NY

Johnson & Johnson Clinical Diagnostics, Inc. - Rochester NY

C12Q 168
C12P 1934

435 5

Nucleic acids can be amplified and detected using an element which has a sealable support on which is disposed a nucleic acid reagent composition. The composition is a mixture of a nucleic acid reagent composed of polymeric particles to which an oligonucleotide is covalently attached. The particles are prepared from a first polymer having a glass transition temperature of at least about 70. degree. C. and have an average diameter of from about 0. 1 to about 3 micrometers. The reagent is adhered to the support using a water insoluble adhesive comprising a second polymer which has a glass transition temperature which is at least about 30. degree. C. less than the glass transition temperature of the first polymer. The adhesive is present in the composition at from about 1 to about 20 dry weight percent. The method provides high sensitivity and low background in the assay of nucleic acids, preferably using polymerase chain reaction.