Inventors:
David Aaron Schwartz - Encinitas CA
William J. Daily - Atascadero CA
Brian Patrick Dwyer - San Diego CA
Kumar Srinivasan - San Diego CA
Bob Dale Brown - Encinitas CA
Assignee:
Promega Biosciences, Inc. - Madison WI
International Classification:
C07C23305
US Classification:
564153, 554 35, 554 36, 554 51, 554 52, 562561, 562563, 564159, 564197
Abstract:
The present invention provides novel amide-based cationic lipids of the general structure: or a salt, or solvate, or enantiomers thereof wherein; (a) Y is a direct link or an alkylene of 1 to about 20 carbon atoms; (b) R is H or a lipophilic moiety; (c) R , R , and R are positively charged moieties, or at least one but not all of R ,R , or R is a positive moiety and the remaining are independently selected from H, an alkyl moiety of 1 to about 6 carbon atoms, or a heterocyclic moiety of about 5 to about 10 carbon atoms; (d) n and p are independently selected integers from 0 to 8, such that the sum of n and o is from 1 to 16; (e) X is an anion or polyanion and (f) m is an integer from 0 to a number equivalent to the positive charge(s) present on the lipid; provided that if Y is a direct link and the sum of n and p is 1 then one of either R or R must have an alkyl moiety of at least 10 carbon atoms. The present invention further provides compositions of these lipids with polyanionic macromolecules, methods for interfering with protein expression in a cell utilizing these compositions and a kit for preparing the same.
